My Math Forum Work out how much volume is required

 Chemistry Chemistry Forum

 June 12th, 2018, 01:27 AM #1 Newbie   Joined: Feb 2016 From: Australia Posts: 18 Thanks: 1 Work out how much volume is required A solution containing diclofenac sodium (MW 381.1 g/mol) at a concentration of 79.5mg/100mL in buffer was prepared. What volume of the solution was required in order to carry out a microsomal incubation containing 25µM of the drug in 1mL? I think we have to use (C1)(V1) = (C2)(V2) Calculating C1: 79.5mg/100mL = 0.795g/L (0.795g/L)/(381.1g/mol)=0.00209mol/L Calculating C2: 25microM = 0.000025mol/L V1 = [(C2)(V2)] / C1 V1 = [(0.000025)(1)] / (0.00209) V1 = 0.0120mL is that correct?
June 12th, 2018, 10:52 AM   #2
Senior Member

Joined: Jun 2015
From: England

Posts: 844
Thanks: 252

Quote:
 Originally Posted by mathmathy A solution containing diclofenac sodium (MW 381.1 g/mol) at a concentration of 79.5mg/100mL in buffer was prepared. What volume of the solution was required in order to carry out a microsomal incubation containing 25µM of the drug in 1mL? I think we have to use (C1)(V1) = (C2)(V2) Calculating C1: 79.5mg/100mL = 0.795g/L (0.795g/L)/(381.1g/mol)=0.00209mol/L Calculating C2: 25microM = 0.000025mol/L V1 = [(C2)(V2)] / C1 V1 = [(0.000025)(1)] / (0.00209) V1 = 0.0120mL is that correct?
I don't think so, but perhaps I am not reading the question correctly.

As I see it, you are find the volume of the stock solution which contains 25 micromoles of diclofenac and make it up to 1ml.

The concentration of diclofenac will then be 25 uM/ml. But this is not the concentration of the stock solution.

Last edited by skipjack; June 12th, 2018 at 09:02 PM.

 June 12th, 2018, 06:24 PM #3 Newbie   Joined: Feb 2016 From: Australia Posts: 18 Thanks: 1 But isn't 25uM = 25umol/L so that is a concentration in itself?

 Tags required, volume, work

 Thread Tools Display Modes Linear Mode

 Similar Threads Thread Thread Starter Forum Replies Last Post integration Computer Science 11 April 18th, 2018 11:35 PM khaliyar Calculus 2 February 24th, 2017 08:24 PM leo255 Calculus 3 October 29th, 2014 02:54 AM mr. jenkins Calculus 5 April 24th, 2014 10:48 AM Useless1 Calculus 2 May 19th, 2008 11:46 AM

 Contact - Home - Forums - Cryptocurrency Forum - Top