June 12th, 2018, 01:27 AM  #1 
Newbie Joined: Feb 2016 From: Australia Posts: 20 Thanks: 1  Work out how much volume is required
A solution containing diclofenac sodium (MW 381.1 g/mol) at a concentration of 79.5mg/100mL in buffer was prepared. What volume of the solution was required in order to carry out a microsomal incubation containing 25µM of the drug in 1mL? I think we have to use (C1)(V1) = (C2)(V2) Calculating C1: 79.5mg/100mL = 0.795g/L (0.795g/L)/(381.1g/mol)=0.00209mol/L Calculating C2: 25microM = 0.000025mol/L V1 = [(C2)(V2)] / C1 V1 = [(0.000025)(1)] / (0.00209) V1 = 0.0120mL is that correct? 
June 12th, 2018, 10:52 AM  #2  
Senior Member Joined: Jun 2015 From: England Posts: 915 Thanks: 271  Quote:
As I see it, you are find the volume of the stock solution which contains 25 micromoles of diclofenac and make it up to 1ml. The concentration of diclofenac will then be 25 uM/ml. But this is not the concentration of the stock solution. Last edited by skipjack; June 12th, 2018 at 09:02 PM.  
June 12th, 2018, 06:24 PM  #3 
Newbie Joined: Feb 2016 From: Australia Posts: 20 Thanks: 1 
But isn't 25uM = 25umol/L so that is a concentration in itself? 

Tags 
required, volume, work 
Thread Tools  
Display Modes  

Similar Threads  
Thread  Thread Starter  Forum  Replies  Last Post 
How digital volume work?  integration  Computer Science  11  April 18th, 2018 11:35 PM 
Calculus 2 : Work Required to Pump Tank of Water  khaliyar  Calculus  2  February 24th, 2017 08:24 PM 
Work required to pump water out of a triangular tank  leo255  Calculus  3  October 29th, 2014 02:54 AM 
Volume Problem (showing lots of work)  mr. jenkins  Calculus  5  April 24th, 2014 10:48 AM 
Help required PLEASE !!  Useless1  Calculus  2  May 19th, 2008 11:46 AM 